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EXAMPLE TEST REPORT #3
Formula Preservation

Antibacterial Time Kill Test

April 1, 1999

Mr./Mrs. Customer
Any Company
Any Town, USA

Dear Mr./Mrs. Customer,

Enclosed please find a copy of our microbiology laboratory report outlining the results of the "Antibacterial Time Kill" test conducted on the following sample:

  • Liquid Dish Detergent/Antibacterial Hand Soap – Containing PCMX

Please feel free to contact me if you have any questions.

Very truly yours,

Donald F. Greene
Microbiologist

SURCIDE PCMX

TECHNICAL SERVICE LABORATORY REPORT
Antibacterial Hand Soap Time Kill Study

REPORT NUMBER: 123-456

COMPANY: Any Company, USA

SAMPLE(S):

  • 1. Liquid Dish Detergent/Antibacterial Hand Soap - Containing PCMX

Contamination Check

The sample was evaluated for initial microbial content and was found to be free of microbial contamination.

Procedure

An inoculum was prepared by mixing 0.25ml of a 24 hour broth culture of the test organisms with 9.75ml of sterile 0.9% saline solution. A serial dilution/viable cell count was prepared on the inoculum to determine the number of organisms/ml of inoculum. One ml of the inoculum was added to 9.0ml of the formulation and mixed with a sterile glass rod. After the approved contact time (60 seconds and 120 seconds), one ml aliquot of the inoculum/product was removed and added to Letheen Neutralizer Broth to neutralize the active ingredient. A second serial dilution/viable cell count was performed on this aliquot to determine the number of surviving organisms. The viable cell counts after 60 and 120 seconds were compared to the initial base line inoculum counts in order to determine the percent (%) of reduction factor (RF). The reduction factor is the % reduction of the challenge organism(s) from the initial baseline population. The RF is calculated as the VCC/Baseline x 100 = X. 100% - X = RF (in %).

Test Organisms

  • Staphylococcus aureus ATCC 6358
  • Escherichia coli ATCC 8739
  • Pseudomonas aeruginosa ATCC 15442

Results

Baseline Exposure
SAMPLE ORGANISM COUNT TIME VCC RF (% REDUCTION)
Liquid Dish/ Antibacterial Hand-Soap E.coli 1,309,000 60 sec 4,000 99.694%
Liquid Dish/ Antibacterial Hand-Soap E.coli 1,309,000 120 sec 1,030 99.921%
Liquid Dish/ Antibacterial Hand-Soap S.aureus 2,600,000 60 sec < 10 > 99.999%
Liquid Dish/ Antibacterial Hand-Soap S.aureus 2,600,000 120 sec < 10 > 99.999%
Liquid Dish/ Antibacterial Hand-Soap Ps.aeruginosa 8,727,000 60 sec 265 99.996%
Liquid Dish/ Antibacterial Hand-Soap Ps.aeruginosa 8,727,000 120 sec < 10 > 99.999%
 
Legend:

VCC – Viable cell count after exposure
RF – Reduction Factor (% reduction from initial baseline count)

 

Donald F. Greene
Microbiologist

April 1, 1999

The information and recommendations set forth are based on our own research and that of others, and to the best of our knowledge believed to be correct. Suggestions made concerning uses or application are only the opinion of Surety Laboratories, Inc. and you should make your own tests to determine the suitability of this information for your own particular purpose. However, because of numerous factors affecting results, Surety Laboratories, Inc. makes no warranty of any kind, expressed or implied. Statements herein therefore, should not be construed as representations or warranties of any kind.